The culture was then split and maintained for 11 days without Ang II. Parasitemia was then evaluated. These results open new avenues for the development of new antimalarial drugs. The reaction of urate with compound II was studied using the same conditions described above. MPO (1 μm) was pre-mixed with 10 μm hydrogen peroxide for a delay of 40 ms. Urate hydroperoxide is likely to exacerbate inflammatory tissue damage and is a possible culprit for the oxygen-dependent inactivation of alcohol dehydrogenase by urate radicals (9). Indeed, it was much more reactive with GSH than hydrogen peroxide. Immunofluorescence staining for prorenin receptor was performed on renal sections from Sprague Dawley rats 2 and 4 wk after inducing diabetes with streptozotocin, a time point when animals were hyperglycemic but had not developed overt nephropathy.
Urate (0–40 μm) was added to the pre-mixing, and the conversion of compound II back to ferric MPO was monitored in the spectroscopic range 380–500 nm from 20 to 200 s and analyzed by monitoring the decay in absorption at 456 nm. Methods 25: 402–408, 2001.OpenUrlCrossRefPubMedWeb of Science ↵ Mifsud SA, Allen TJ, Bertram JF, Hulthen UL, Kelly DJ, Cooper ME, Wilkinson-Berka JL, Gilbert RE. Podocyte foot process broadening in experimental diabetic nephropathy: amelioration with renin-angiotensin blockade. Administration of ANG-(1–7) resulted in a significant increase in plasma renin activity by 2.3-fold (P < 0.05). Similarly, glomerular renin mRNA expression did not differ between nephritic rats and normal rats.
Mass spectrometry analyses were performed using an Applied Biosystems 4000QTrap (Concord, Ontario, Canada). The electrospray needle was held at 400 °C. Nitrogen was both the curtain and collision gas. After 20 min, catalase was added (100 μg/ml) to consume hydrogen peroxide, and organic hydroperoxides were quantified by the FOX assay. Mounting evidence suggests that the podocyte is a direct target of ANG II-mediated signaling in diabetic renal disease. We have tested the hypothesis that high glucose leads to the activation of a local angiotensin system in podocytes and delineated the underlying pathways involved. Genes and antigens of parasites: a laboratory manual proceedings. Our data do not exclude the possibility that electron transfer from superoxide also occurs to reform urate. For unlabeled allantoin, the largest fragment ion generated by collision-induced disassociation of the [M − H]− ion was used for quantification, and a second fragment ion was used for confirmation.